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KMID : 0368419990420040317
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Journal of Plant Biology
1999 Volume.42 No. 4 p.317 ~ p.320
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Construction of Small Binary Vectors for Agrobacterium Mediated Transformation in Plants
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Lim, Jeong Hwa
Kang, Young Na/Kim, Young A/Kim, Dae Heon/Hwang, Inhwan
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Abstract
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Despite the popular use of the Agrobacterium-mediated method for transforming plants, only a very limited number of binary vectors are available. In addition, those binary vectors are rather inconvenient for plasmid manipulation because of their large size. Here, we report the construction of small binary vectors, pBmin1 and pBminGFP1, for Agrobacterium-mediated transformation. The sizes of pBmin1 and pBminGFP1 are approximately 5.0kb and 6.5kb, respectively. These vectors contain one kanamycin resistance gene, with a double promoter for expression in both bacteria and plant cells. Vector pBminGFP1 has the 35S-GFP chimeric gene as a reporter. These small binary vectors would greatly facilitate plasmid manipulation and plant transformation.
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